In this lab experiment, we do protein assays by using Biuret assay method to determine the protein concentration. The concentration of protein is measured by the wavelength of 540nm, A540, by using spectrophotometer. At first, we mix 1mg/ml of 0.50ml of gelatin (protein) with 2.5ml of Biuret reagent into the cuvette. Then, we repeat the first step by replacing 1mg/ml of 0.50ml of gelatin by 2mg/ml, 3mg/ml, 4mg/ml, 5mg/ml and 6mg/ml of 0.50ml of gelatin into other prepared cuvettes. Then we put each cuvette with different protein concentration into the spectrophotometer and the results are recorded in the table below.
Graph 1: The absorbance of light at 540nm versus the different gelatin concentration.
From the experiment result, I found that concentration of protein in X (duck egg) is 18.67mg/L while in grade A egg is 14.23mg/L. Duck egg has more 23.8% protein content compared to grade A egg. Although duck egg has more protein content and is good for our health but if we compare the cost we found that a duck egg is more expensive than a grade A egg. The duck egg in market is RM 1.00 and grade A egg is RM 0.34. If we spend RM 1.00 to buy grade A eggs that’s mean we will obtain more protein compare to spend RM 1.00 to buy only a duck egg. At here I prefer to choose grade A egg.
When I compare the protein content in grade A egg and grade C egg, I found that grade C egg has a concentration of protein 10.95mg/L. Grade A egg has more 30.0% of protein content compared to grade C egg. In market, a grade C egg is RM 0.30 while grade A egg is RM 0.34. Since grade A egg is more larger and has more protein content than grade C egg, so, between A and C I will choose to buy grade A egg.
Finally, I compare the grade A and grade B egg and I found that grade A egg concentration protein is 14.23mg/L while grade B egg concentration protein is 16.38mg/L, which is higher 2.15mg/L than grade A egg. When I get the data I feel surprise because at first I guess grade A egg has higher protein content than grade B egg. Since the cost different between grade A and B eggs is RM 0.02 but grade B egg has higher protein content. As a result, I will choose to buy grade B egg.
As a conclusion, I can say that the grade of an egg is not enough to determine the protein content in it. As a wise consumer we should choose to buy grade B egg because it has more protein content-16.38mg/L, more valuable and the prize is moderate-RM 0.32 each.
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